Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry.
نویسندگان
چکیده
Dissociation of disulfide is normally mandatory prior to disulfide peptide sequencing via electrospray ionization collision induced dissociation mass spectrometry (ESI-CID-MS). Herein, a facile method for directly sequencing intact disulfide peptides was proposed. The basic principles involved were electrolyte-enhanced corona discharge in ESI and the following oxidative cleavage reaction.
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ورودعنوان ژورنال:
- The Analyst
دوره 140 8 شماره
صفحات -
تاریخ انتشار 2015